In drugresistant epilepsy patients, stem cell therapy shows. Cerebral palsy cp is a syndrome of childhood movement and posture disorders. The immunomodulatory properties of mesenchymal stem cells and. Aug 31, 2016 mesenchymal stem stromal cells as a delivery platform in cell and gene therapies regenerative medicine relying on cell and gene therapies is one of the most promising approaches to repair tissues.
Bone marrow mesenchymal stem cells bmmscs and bone marrow mononuclear cells bmmncs are both used to treat spastic cerebral palsy. The cell body contains a large, round nucleus with a prominent nucleolus, which is surrounded by finely dispersed chromatin particles, giving the nucleus a clear appearance. Bone marrow stromal cells mesenchymal stem cells experimental models nervous system stem cell transplantation tissue regeneration neural stem cell cell transplantation abstract mononuclear cells mncs and mesenchymal stem cells mscs derived from the bone. Cell therapy bone marrow mesenchymal stem cells drugresistant epilepsy transplantation. Pdf stem cell therapy for treatment of epilepsy researchgate. Mesenchymal stem cells, however, isolated from bone marrow and other tissues have been engineered to release neuroprotective and potential seizureattenuating factors in epilepsy e. Novel approaches based on stem cell therapy offer the potential for curing epilepsy, rather than treating the symptoms. Xenograft of human umbilical mesenchymal stem cells from. Huntingtons disease hd is caused by an expansion of polyq repeats within the amino terminus of the huntingtin htt protein, which promotes htt aggregation and formation of intracellular inclusion bodies. Therapeutic evidence of umbilical cordderived mesenchymal. Cell therapy bone marrow mesenchymal stem cells drugresistant epilepsy transplantation abstract there is a need among patients suffering from drugresistant epilepsy.
Various stem cell types were used for treatment of epilepsy in basic and. Epilepsy therapy is largely symptomatic and no effective therapy is available to prevent epileptogenesis. Therapeutic potential of mesenchymal stem cellderived. Temporal lobe epilepsy tle is present in 30% of epileptic patients and does not respond to conventional treatments. Engineered adenosinereleasing cells for epilepsy therapy. Mesenchymal stem cellderived exosomes mscexo have robust antiinflammatory effects in the treatment of neurological diseases such as epilepsy, stroke, or traumatic brain injury. Research open access transplantation of human cord blood. Mesenchymal stromal cells mscs are a heterogeneous population of cells that can be derived from multiple tissue types in the body. Stem cell therapy may become a viable treatment option for epilepsy. An open label study article pdf available in advances in medical sciences 622. As most cases of epilepsy can be attributed to receptor expression differences within the brain due to mutations, correcting these may in theory reduce the likelihood of electrical seizures developing in the brain.
The remainder of the cell body contains a small amount of golgi apparatus, rough endoplasmic. Jul 05, 2019 please use one of the following formats to cite this article in your essay, paper or report. Hindwai publishing corporation stem cell international, 2012. In addition, veeg recording was performed during chronic epilepsy period between 6 and9 weeks post. May 15, 2017 type 2 diabetes mellitus t2dm, which is characterized by the combination of relative insulin deficiency and insulin resistance, cannot be reversed with existing therapeutic strategies. Given their ability to proliferate, differentiate and regenerate tissues, bone marrow derived mesenchymal stem cells bmscs could restore neural circuits lost during the course of the disease and reestablish the physiological. But isolating the tiny fraction of cells that are mesenchymal stem cells is more complicated. It is triggered by a mutation in the htt gene that strongly influences functional abilities and usually results in movement, cognitive and psychiatric disorders. Mesenchymal stem cells in cancer therapy 1st edition.
The aim of this paper was to describe the outcome of the therapeutic administration of allogenic mesenchymal stem cells obtained from whartons jelly wjmscs in children with cerebral palsy cp during a medical therapeutic experiment. Condition or disease, interventiontreatment, phase. Frontiers mesenchymal stem cells in the treatment of. The present study addresses the feasibility of bmscs transplantation against pilocarpineinduced tle experimentally. Safety of autologous msc infusion to treat epilepsy full. Intrathecal infusion of autologous adiposederived regenerative. Mesenchymal stem cells for therapeutic model of epilepsy. Autologous mesenchymal stem cells, phase 1 phase 2. Epilepsy is a common and devastating health problem that affects 6. Mesenchymal stem cells are a distinct entity to the mesenchyme, embryonic connective tissue which is derived from the mesoderm and differentiates to form hematopoietic stem cells. Multipotent mesenchymal stem stromal cells msc, a population of progenitors committing into. Human umbilical cord mesenchymal stem cells hucmscs are attractive because of their easy accessibility, low immunogenicity, and immunosuppressive potential compared with other types of stem cells.
While the basic biology of mscs continues to be investigated, their ability to modulate the immune response and to enhance tissue regeneration and repair has led to a substantial number of clinical investigations assessing the therapeutic potential of mscs for a. Please use one of the following formats to cite this article in your essay, paper or report. Administration of mesenchymal stem cell mscderived secretomes shows therapeutic potential as effects similar to those observed after transplantation of mscs have been reported. Human induced pluripotent stem cellderived mge cell grafting after status epilepticus attenuates chronic epilepsy and comorbidities via synaptic integration. Cell therapy for the treatment of epilepsy comprises a few primary.
Untuk itu penulis mengucapkan terima kasih kepada dr. The study of these mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self. Combined adipose tissuederived mesenchymal stem cell. Human umbilical cord bloodderived mesenchymal stem cell transplantation attenuates severe brain injury by permanent middle cerebral artery occlusion in newborn rats. Safety of autologous msc infusion to treat epilepsy full text view. Autologous mscs may be a better cell source and have been studied for the treatment of cerebral palsy because of their functions in tissue repair and the regulation of immunological processes. Human mesenchymal stem cells and human embryonic stem cells. We conducted a randomized trial to evaluate the safety and efficacy of hucmsc transplantation concomitant with rehabilitation in patients with cp. Human mesenchymal stem cells and human embryonic stem cells detlev boison grid. Hd is incurable, although treatments are available to help manage symptoms and to delay the physical, mental and behavioral declines. Pdf treatment of refractory epilepsy patients with autologous.
Stem cell therapy is a promising treatment for cerebral palsy, which refers to a category of brain diseases that are associated with chronic motor disability in children. Effect of neural stem cellsprogenitor cells and mesenchymal stem cell transplantation on huntingtons disease etiology and progression. Multiple autologous bone marrowderived cd271 1 mesenchymal stem cell transplantation overcomes drugresistant epilepsy in children olga milczarek,a danuta jarocha,b anna starowiczfilip,c stanislaw kwiatkowski,a bogna badyra,b marcin majka b key words. We retrospectively analyzed the records of 109 patients recruited in daily clinical practice. In addition, during status epilepticus, the excitation overload sometimes kills neurons. Transplantation of insulinproducing cells ipcs was once thought to be the most promising strategy for treating diabetes, but the pace from the laboratory to clinical application has been obstructed due to. Jul 14, 2015 efficacy of autologous bone marrowderived mesenchymal stem cells in patients with drugresistant symptomatic epilepsy time frame. Although antiepileptic medications can control seizures in many patients, 3040% of people with epilepsy are refractory to medical therapy kwan and brodie, 2000. The majority of patients with intractable epilepsy have partial epilepsy. Mesenchymal stem cells in cancer therapy sheds light on current stem cell based targeted therapies for cancer, by focusing on the application of mesenchymal stem cells msc in various cancers with emphasis on a number of aspects that are critical to the success of future stem cell based therapies for cancer. Mesenchymal stem cell therapy in type 2 diabetes mellitus. Drugresistant epilepsy in children olga milczarek,a danuta jarocha,b anna starowiczfilip,c stanislaw kwiatkowski,a bogna badyra,b marcin majka b key words. Efficacy of autologous bone marrowderived mesenchymal stem cells in patients with drugresistant symptomatic epilepsy time frame.
Research open access transplantation of human cord. Bone marrow derived mesenchymal stem cells transplantation as. A total of 105 patients with spastic cerebral palsy were enrolled and randomly assigned to three groups. It is fairly easy to obtain a mixture of different mesenchymal cell types from adult bone marrow for research. Under the directions of sri estuningsih, arief boediono, and nurhadi ibrahim. Morphologically, mesenchymal stem cells have long thin cell bodies with a large nucleus. Research open access transplantation of human cord blood mononuclear cells and umbilical cordderived mesenchymal stem cells in autism yongtao lv1, yun zhang2, min liu1, jianati qiuwaxi2, paul ashwood3, sungho charles cho4, ying huan1, rucun ge1, xingwang chen1, zhaojing wang2, byungjo kim5 and xiang hu2 abstract. Whartons jelly mesenchymal stem cell administration improves. The immunomodulatory properties of mesenchymal stem. Human mesenchymal stem cells hmscs were engineered to release adenosine using a lentivirus coexpressing emerald green fluorescent protein emgfp and mirna directed against the major adenosineremoving enzyme adenosine kinase adk. May 16, 2018 human umbilical cord bloodderived mesenchymal stem cell transplantation attenuates severe brain injury by permanent middle cerebral artery occlusion in newborn rats. Pdf treatment of refractory epilepsy patients with. Treatment of refractory epilepsy patients with autologous.
Suppression of kindling epileptogenesis by adenosine. Therapeutic potential of mesenchymal stem cellderived secretomes in. Intracerebral delivery of the m2 polarizing cytokine. Bone marrow derived mesenchymal stem cells bmscs induce endogenous neural stem cells, inhibit neurodegeneration, and promote brain selfrepair mechanisms. Huntingtons disease hd is an inherited disease that causes progressive nerve cell degeneration. The resulting adkknockdown cells h239 were used in the present study. Recently, stem cell intervention has shed light on the treatment strategy targeting a cure. Clinical evidence is still limited and sometimes inconclusive about the benefits of human umbilical cord mesenchymal stem cells hucmscs for cp. Mesenchymal stem cell products for every facet of discovery mesenchymal stem cells mscs are fibroblastlike cells isolated from bone marrow, adipose, and other tissues including cord blood, peripheral blood, fetal liver, skeletal muscle, placenta, amniotic.
Mesenchymal stem cell transition to tumorassociated fibroblasts contributes to fibrovascular network expansion and tumor progression. Mesenchymal stem cells mscs also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts bone cells, chondrocytes cartilage cells, myocytes muscle cells and adipocytes fat cells which give rise to marrow adipose tissue. Toxic mutations in dnm1, a gene involved in the proper functioning of brain cells, cause developmental and epileptic encephalopathya severe class of epilepsy with no cure. This is because these approaches have the potential to curb epileptogenesis and prevent chronic epilepsy development and learning and memory dysfunction after hippocampal. However, the differences in therapeutic effect remain unknown. Migration assays for tracing and deducing cell migration.
Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin. Type 2 diabetes mellitus t2dm, which is characterized by the combination of relative insulin deficiency and insulin resistance, cannot be reversed with existing therapeutic strategies. Aimiuwu and colleagues show that a single gene therapy treatment via rna interference eliminated the toxic allele sufficiently to curb severe lethal seizures, eliminate motor and developmental deficits, and extend survival. Stem cell, mesenchymal stem cells msc, msc proliferation and differentiation introduction in the past decade, the field of stem cell biology has undergone a remarkable evolution sparked by report demonstrating that adult stem cells possess greater plasticity than dictated by established paradigms of embryonic development. The immunomodulatory properties of mesenchymal stem cells and their use for immunotherapy martin j hoogduijna. While astrocytes are thought to be mediators of these effects, their precise role remains poorly understood. We report the use of autologous patientderived mesenchymal stem cells msc as a therapeutic agent in symptomatic drugresistant epilepsy in a phase i open. Nextgeneration stem cells ushering in a new era of cell. New prospects of mesenchymal stem cells for ameliorating. Mesenchymal stem cells are multipotent stromal cells that can be extracted from virtually any adult tissue and have the potential to differentiate into a variety of cell types including the osteogenic, adipogenic, chondrogenic, and neural lineages 7275 figure 2. They uncover changes in cell fate potential upon nsc activation, but it comes at a cost. Temporal lobe epilepsy tle, characterized by hyperexcitability in the hippocampus and spontaneous seizures, is a possible clinical target for stem cellbased therapies.
The animal modelbased body of evidence in favor of stem cell therapy for epilepsy is rapidly accumulating. Temporal lobe epilepsy tle, characterized by hyperexcitability in the hippocampus and spontaneous seizures, is a possible clinical target for stem cell based therapies. Mesenchymal stem cell an overview sciencedirect topics. Human induced pluripotent stem cell derived mge cell grafting after status epilepticus attenuates chronic epilepsy and comorbidities via synaptic integration. Jan 26, 20 to assess neural stem celllike nsclike cells derived from autologous marrow mesenchymal stem cells as a novel treatment for patients with moderatetosevere cerebral palsy, a total of 60 cerebral palsy patients were enrolled in this openlabel, nonrandomised, observerblinded controlled clinical study with a 6months followup. Mesenchymal stem cell products for every facet of discovery mesenchymal stem cells mscs are fibroblastlike cells isolated from bone marrow, adipose, and other tissues including cord blood, peripheral blood, fetal liver, skeletal muscle, placenta, amniotic fluid and synovium. The present study addresses the feasibility of bmscs transplantation against pilocarpineinduced tle. We therefore analysed the potential of stem cell derived brain implants and of paracrine adenosine release to suppress the progressive development of seizures in the rat kindlingmodel. We report the use of autologous patientderived mesenchymal stem cells msc as a therapeutic agent in symptomatic drugresistant epilepsy in a phase i open label clinical trial registered as. Autism, cord blood mononuclear cell, umbilical cordderived mesenchymal stem cell, cell transplantation correspondence. They can be harvested easily from the host bone marrow and do. Neural and mesenchymal stem cells in animal models of.
Comparative analysis of curative effect of bone marrow. Mscderived extracellular vesicles evs, containing trophic and immunomodulatory factors, rapidly diffuse throughout the tissue, successfully attenuating tissue. Numerous animal model studies have demonstrated that intracerebral gene and neural cell therapies in acute and chronic models of epilepsy have promise for providing neuroprotection, facilitating neural repair, inducing anti. Neural stem celllike cells derived from autologous bone. Human mesenchymal cells from whartons jelly of the umbilical cord are obtained from medical waste after delivery and therefore carry little ethical concerns.
Bone marrow derived mesenchymal stem cells transplantation. The study is intended for patients after allogeneic hematopoietic stem cell transplantation from related and unrelated donors with steroidrefractory or steroid. Treatment of refractory epilepsy patients with autologous mesenchymal stem cells reduces seizure frequency. Multiple transplantations were performed, causing no adverse events during 2 years of followup. Penelitian ini penulis selesaikan atas berbagai sumbangan pemikiran dan masukan dari komisi pembimbing serta bantuan pihak lain. Human mesenchymal stem cell grafts engineered to release. Epilepsy is a chronic neurologic disease, caused by abnormal electrical. They can be harvested easily from the host bone marrow and do not cause any immunological problems. Given their ability to proliferate, differentiate and regenerate tissues, bone marrow derived mesenchymal stem cells bmscs could restore neural circuits lost during the course of the disease and reestablish the. Whartons jelly mesenchymal stem cell administration.
However, little is known on the exact trophic mechanisms of stem cell therapy in epilepsy. This is because these approaches have the potential to curb epileptogenesis and prevent chronic epilepsy development and learning and memory dysfunction after hippocampal damage related to status epilepticus or head injury. We therefore analysed the potential of stem cellderived brain implants and of paracrine adenosine release to suppress the progressive development of. The utilization of mesenchymal stem cells msc for the treatment of graft versus host disease gvhd after allogeneic stem cell transplantation.
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